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Effect of trifluoroethanol on antibody reactivity against corresponding and nonrelated antigens
- Source :
- The Ukrainian Biochemical Journal, Vol 90, Iss 4, Pp 80-89 (2018)
- Publication Year :
- 2018
- Publisher :
- National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry, 2018.
-
Abstract
- The ability of antibodies to switch between specific and nonspecific recognition of antigens under various factors is the key issue. Here we demonstrate that 2,2,2-trifluoroethanol (TFE) is one of these factors influencing the ability of monoclonal antibodies to react specifically with corresponding antigen (ovalbumin) and transforming them into polyreactive immunoglobulins (PRIGs) that are strong but nonspecific binders with various antigens. Such switching of antibody reactivity is nonlinear and even nonmonotonous function of TFE concentration and depends strongly on incubation time and temperature. At room temperatures (25 °C) the specific antibodies under 30% TFE action are transformed into PRIGs. However, at 0 °C the variation of antibody reactivity is complicated. TFE is known as the alcohol with one of the strongest proton-donor abilities in hydrogen bonding and its effect is probably in binding to specific sites that switch the antibody recognition ability.
Details
- Language :
- English
- ISSN :
- 24094943 and 24135003
- Volume :
- 90
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- The Ukrainian Biochemical Journal
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.381ba7195afd4c07ab606de84dc42a40
- Document Type :
- article
- Full Text :
- https://doi.org/10.15407/ubj90.04.080