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Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration

Authors :
Mao‐yuan Wang
Jia‐ming Yang
Yi Wu
Hai Li
Yan‐biao Zhong
Yun Luo
Rui‐lian Xie
Source :
Journal of Cachexia, Sarcopenia and Muscle, Vol 15, Iss 5, Pp 1834-1849 (2024)
Publication Year :
2024
Publisher :
Wiley, 2024.

Abstract

Abstract Background Skeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel. Methods Myogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining. Results During myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P

Details

Language :
English
ISSN :
21906009, 21905991, and 38096579
Volume :
15
Issue :
5
Database :
Directory of Open Access Journals
Journal :
Journal of Cachexia, Sarcopenia and Muscle
Publication Type :
Academic Journal
Accession number :
edsdoj.38096579089f47e1961aa158de00e0be
Document Type :
article
Full Text :
https://doi.org/10.1002/jcsm.13535