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The protective effects of Xuebijing injection on intestinal injuries of mice exposed to irradiation

Authors :
Yinping Dong
YuanYang Zhang
Xinyue Wang
Wenxuan Li
Junling Zhang
Lu Lu
Hui Dong
Saijun Fan
Aimin Meng
Deguan Li
Source :
Animal Models and Experimental Medicine, Vol 5, Iss 6, Pp 565-574 (2022)
Publication Year :
2022
Publisher :
Wiley, 2022.

Abstract

Abstract Background Gastrointestinal (GI) injury is one of the most common side effects of radiotherapy. However, there is no ideal therapy method except for symptomatic treatment in the clinic. Xuebijing (XBJ) is a traditional Chinese medicine, used to treat sepsis by injection. In this study, the protective effects of XBJ on radiation‐induced intestinal injury (RIII) and its mechanism were explored. Methods The effect of XBJ on survival of irradiated C57BL/6 mice was monitored. Histological changes including the number of crypts and the length of villi were evaluated by H&E. The expression of Lgr5+ intestinal stem cells (ISCs), Ki67+ cells, villin and lysozymes were examined by immunohistochemistry. The expression of cytokines in the intestinal crypt was detected by RT‐PCR. DNA damage and apoptosis rates in the small intestine were also evaluated by immunofluorescence. Results In the present study, XBJ improved the survival rate of the mice after 8.0 and 9.0 Gy total body irradiation (TBI). XBJ attenuated structural damage of the small intestine, maintained regenerative ability and promoted proliferation and differentiation of crypt cells, decreased apoptosis rate and reduced DNA damage in the intestine. Elevation of IL‐6 and TNF‐α was limited, but IL‐1, TNF‐𝛽 and IL‐10 levels were increased in XBJ‐treated group after irradiation. The expression of Bax and p53 were decreased after XBJ treatment. Conclusions Taken together, XBJ provides a protective effect on RIII by inhibiting inflammation and blocking p53‐related apoptosis pathway.

Details

Language :
English
ISSN :
25762095
Volume :
5
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Animal Models and Experimental Medicine
Publication Type :
Academic Journal
Accession number :
edsdoj.37a858cf13d547aab7c7520bf8236c52
Document Type :
article
Full Text :
https://doi.org/10.1002/ame2.12285