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Identification of c-MYC SUMOylation by mass spectrometry.

Authors :
Manpreet Kalkat
Pak-Kei Chan
Amanda R Wasylishen
Tharan Srikumar
Sam S Kim
Romina Ponzielli
David P Bazett-Jones
Brian Raught
Linda Z Penn
Source :
PLoS ONE, Vol 9, Iss 12, p e115337 (2014)
Publication Year :
2014
Publisher :
Public Library of Science (PLoS), 2014.

Abstract

The c-MYC transcription factor is a master regulator of many cellular processes and deregulation of this oncogene has been linked to more than 50% of all cancers. This deregulation can take many forms, including altered post-translational regulation. Here, using immunoprecipitation combined with mass spectrometry, we identified a MYC SUMOylation site (K326). Abrogation of signaling through this residue by substitution with arginine (K326R) has no obvious effects on MYC half-life, intracellular localization, transcriptional targets, nor on the biological effects of MYC overexpression in two different cell systems assessed for soft agar colony formation, proliferation, and apoptosis. While we have definitively demonstrated that MYC SUMOylation can occur on K326, future work will be needed to elucidate the mechanisms and biological significance of MYC regulation by SUMOylation.

Subjects

Subjects :
Medicine
Science

Details

Language :
English
ISSN :
19326203
Volume :
9
Issue :
12
Database :
Directory of Open Access Journals
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
edsdoj.36543a850d5a4a229521ec2c61e6d4e2
Document Type :
article
Full Text :
https://doi.org/10.1371/journal.pone.0115337