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Prediction model for day 3 embryo implantation potential based on metabolites in spent embryo culture medium

Authors :
Rong Liang
Sheng Nan Duan
Min Fu
Yu Nan Chen
Ping Wang
Yuan Fan
Shihui Meng
Xi Chen
Cheng Shi
Source :
BMC Pregnancy and Childbirth, Vol 23, Iss 1, Pp 1-9 (2023)
Publication Year :
2023
Publisher :
BMC, 2023.

Abstract

Abstract Background Metabolites in spent embryo culture medium correlate with the embryo’s viability. However, there is no widely accepted method using metabolite dada to predict successful implantation. We sought to combine metabolomic profiling of spent embryo culture medium and clinical variables to create an implantation prediction model as an adjunct to morphological screening of day 3 embryos. Methods This investigation was a prospective, nested case-control study. Forty-two day 3 embryos from 34 patients were transferred, and the spent embryo culture medium was collected. Twenty-two embryos implanted successfully, and the others failed. Metabolites in the medium relevant to implantation were detected and measured by Liquid Chromatography-Mass Spectrometry. Clinical signatures relevant to embryo implantation were subjected to univariate analysis to select candidates for a prediction model. Multivariate logistical regression of the clinical and metabolomic candidates was used to construct a prediction model for embryo implantation potential. Results The levels of 13 metabolites were significantly different between the successful and failed groups, among which five were most relevant and interpretable selected by Least Absolute Shrinkage and Selection Operator regression analysis. None of the clinical variables significantly affected day 3 embryo implantation. The most relevant and interpretable set of metabolites was used to construct a prediction model for day 3 embryo implantation potential with an accuracy of 0.88. Conclusions Day 3 embryos’implantation potential could be noninvasively predicted by the spent embryo culture medium’s metabolites measured by LC-MS. This approach may become a useful adjunct to morphological evaluation of day 3 embryos.

Details

Language :
English
ISSN :
14712393
Volume :
23
Issue :
1
Database :
Directory of Open Access Journals
Journal :
BMC Pregnancy and Childbirth
Publication Type :
Academic Journal
Accession number :
edsdoj.3636b495d6094adfbb474fe5062f0c48
Document Type :
article
Full Text :
https://doi.org/10.1186/s12884-023-05666-7