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Curcumin enhances liver SIRT3 expression in the rat model of cirrhosis
- Source :
- Iranian Journal of Basic Medical Sciences, Vol 20, Iss 12, Pp 1306-1311 (2017)
- Publication Year :
- 2017
- Publisher :
- Mashhad University of Medical Sciences, 2017.
-
Abstract
- Objective(s): Bill duct ligation (BDL) is a representative model of biliary cholestasis in animals. Curcumin has a protective effect on the liver; however, its underlying mechanisms are not completely known. This study explored the hepatoprotective activity of curcumin on hepatic damage via measuring the expression of sirtuin3 (SIRT3), AMP-activated protein kinase (AMPK), carnitine palmitoyltransferase 1A (CPT-1A), isocitrate dehydrogenase2 (IDH2) and manganese superoxide dismutase (MnSOD) as well as the level of serum lipid profile in the BDL fibrotic rat model. Materials and Methods: The study consisted of four groups (n=8 for each of Wistar rats): sham group, sham+curcumin (sham+Cur) group (received curcumin 100 mg/kg/day), BDL+Cur group, and BDL group. Transcription levels of SIRT3, AMPK, CPT-1A, IDH2, MnSOD and protein expression level of SIRT3 were measured by real-time PCR and Western blotting, respectively. Results: It was identified that SIRT3, AMPK, CPT-1A, IDH2 and MnSOD expression significantly decreased in BDL rats compared to sham rats; however, in the curcumin treatment of BDL rats, the expression of these factors increased significantly compared to BDL (P-value
- Subjects :
- Curcumin
Gene expression
Liver Cirrhosis
Rats
SIRT3 protein
Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 20083866 and 20083874
- Volume :
- 20
- Issue :
- 12
- Database :
- Directory of Open Access Journals
- Journal :
- Iranian Journal of Basic Medical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.35a6cec2d32b441081788758b374cc75
- Document Type :
- article
- Full Text :
- https://doi.org/10.22038/ijbms.2017.9609