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EFFECT OF A NOVEL HEAT SHOCK PROTEIN 90/HISTONE DEACETYLASE DUAL-TARGET INHIBITOR ON THE PROLIFE-RATION, INVASION, AND MIGRATION OF BREAST CANCER MDA-MB-231 CELLS AND ITS MECHANISM
- Source :
- 精准医学杂志, Vol 38, Iss 3, Pp 249-253 (2023)
- Publication Year :
- 2023
- Publisher :
- Editorial Office of Journal of Precision Medicine, 2023.
-
Abstract
- Objective To investigate the effect of FXX-W-12-4, a novel heat shock protein 90 (Hsp90)/histone deacetylase (HDAC) dual-target inhibitor, on the proliferation, invasion, and migration of breast cancer MDA-MB-231 cells. Methods CCK-8 assay was used to observe the effect of FXX-W-12-4 on the viability of MDA-MB-231 cells, and its half-maximal inhibitory concentration (IC50) was calculated as the reference value for concentration in follow-up experiment. On the basis of the IC50 value, FXX-W-12-4 with a final concentration of 0, 100, 200, and 300 nmol/L was added to MDA-MB-231 cells and was established as groups A, B, C, and D, respectively. Cell scratch assay and Transwell assay were used to observe the effect of FXX-W-12-4 on the invasion and migration of MDA-MB-231 cells in each group. Western blot was used to measure the relative expression levels of proteins such as ac-H3 and Hsp70 in MDA-MB-231 cells. Results CCK-8 assay showed that the viability of MDA-MB-231 cells gradually decreased with the increase in FXX-W-12-4 concentration (F=2 663.00,P
Details
- Language :
- Chinese
- ISSN :
- 2096529X
- Volume :
- 38
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- 精准医学杂志
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.34fa410c38c04cc8866392a12d60af19
- Document Type :
- article
- Full Text :
- https://doi.org/10.13362/j.jpmed.202303014