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Detection of 1,5-anhydroglucitol as a Biomarker for Diabetes Using an Organic Field-Effect Transistor-Based Biosensor

Authors :
Hiroyuki Furusawa
Yusuke Ichimura
Kuniaki Nagamine
Rei Shiwaku
Hiroyuki Matsui
Shizuo Tokito
Source :
Technologies, Vol 6, Iss 3, p 77 (2018)
Publication Year :
2018
Publisher :
MDPI AG, 2018.

Abstract

Sensor devices that can be fabricated on a flexible plastic film produced at a low cost using inkjet-printing technology are suitable for point-of-care applications. An organic field-effect transistor (OFET)-based biosensor can function as a potentiometric electrochemical sensor. To investigate the usefulness of an OFET-based biosensor, we demonstrated the detection of 1,5-anhydroglucitol (1,5-AG) and glucose, which are monosaccharides used as biomarkers of diabetes. An OFET-based biosensor combined with a Prussian blue (PB) electrode, modified with glucose oxidase (GOx) or pyranose oxidase (POx), was utilized for the detection of the monosaccharides. When the GOx- or POx-PB electrode was immersed in glucose solution at the determined concentration, shifts in the low-voltage direction of transfer characteristic curves of the OFET were observed to be dependent on the glucose concentrations in the range of 0–10 mM. For 1,5-AG, the curve shifts were observed only with the POx-PB electrode. Detection of glucose and 1,5-AG was achieved in a substrate-specific manner of the enzymes on the printed OFET-biosensor. Although further improvements are required in the detection concentration range, the plastic-filmOFET-biosensors will enable the measurement of not only diabetes biomarkers but also various other biomarkers.

Details

Language :
English
ISSN :
22277080
Volume :
6
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Technologies
Publication Type :
Academic Journal
Accession number :
edsdoj.34de5df7075b41908a9e5899ff67f3ae
Document Type :
article
Full Text :
https://doi.org/10.3390/technologies6030077