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Detection of 1,5-anhydroglucitol as a Biomarker for Diabetes Using an Organic Field-Effect Transistor-Based Biosensor
- Source :
- Technologies, Vol 6, Iss 3, p 77 (2018)
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Sensor devices that can be fabricated on a flexible plastic film produced at a low cost using inkjet-printing technology are suitable for point-of-care applications. An organic field-effect transistor (OFET)-based biosensor can function as a potentiometric electrochemical sensor. To investigate the usefulness of an OFET-based biosensor, we demonstrated the detection of 1,5-anhydroglucitol (1,5-AG) and glucose, which are monosaccharides used as biomarkers of diabetes. An OFET-based biosensor combined with a Prussian blue (PB) electrode, modified with glucose oxidase (GOx) or pyranose oxidase (POx), was utilized for the detection of the monosaccharides. When the GOx- or POx-PB electrode was immersed in glucose solution at the determined concentration, shifts in the low-voltage direction of transfer characteristic curves of the OFET were observed to be dependent on the glucose concentrations in the range of 0–10 mM. For 1,5-AG, the curve shifts were observed only with the POx-PB electrode. Detection of glucose and 1,5-AG was achieved in a substrate-specific manner of the enzymes on the printed OFET-biosensor. Although further improvements are required in the detection concentration range, the plastic-filmOFET-biosensors will enable the measurement of not only diabetes biomarkers but also various other biomarkers.
Details
- Language :
- English
- ISSN :
- 22277080
- Volume :
- 6
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Technologies
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.34de5df7075b41908a9e5899ff67f3ae
- Document Type :
- article
- Full Text :
- https://doi.org/10.3390/technologies6030077