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Photostable and Biocompatible Fluorescent Silicon Nanoparticles for Imaging-Guided Co-Delivery of siRNA and Doxorubicin to Drug-Resistant Cancer Cells
- Source :
- Nano-Micro Letters, Vol 11, Iss 1, Pp 1-13 (2019)
- Publication Year :
- 2019
- Publisher :
- SpringerOpen, 2019.
-
Abstract
- Abstract The development of effective and safe vehicles to deliver small interfering RNA (siRNA) and chemotherapeutics remains a major challenge in RNA interference-based combination therapy with chemotherapeutics, which has emerged as a powerful platform to treat drug-resistant cancer cells. Herein, we describe the development of novel all-in-one fluorescent silicon nanoparticles (SiNPs)-based nanomedicine platform for imaging-guided co-delivery of siRNA and doxorubicin (DOX). This approach enhanced therapeutic efficacy in multidrug-resistant breast cancer cells (i.e., MCF-7/ADR cells). Typically, the SiNP-based nanocarriers enhanced the stability of siRNA in a biological environment (i.e., medium or RNase A) and imparted the responsive release behavior of siRNA, resulting in approximately 80% down-regulation of P-glycoprotein expression. Co-delivery of P-glycoprotein siRNA and DOX led to > 35-fold decrease in the half maximal inhibitory concentration of DOX in comparison with free DOX, indicating the pronounced therapeutic efficiency of the resultant nanocomposites for drug-resistant breast cancer cells. The intracellular time-dependent release behaviors of siRNA and DOX were revealed through tracking the strong and stable fluorescence of SiNPs. These data provide valuable information for designing effective RNA interference-based co-delivery carriers.
- Subjects :
- Fluorescent silicon nanoparticles
Drug resistance
Gene therapy
Bioimaging
Technology
Subjects
Details
- Language :
- English
- ISSN :
- 23116706 and 21505551
- Volume :
- 11
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Nano-Micro Letters
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.345fdd35a3284410b3e4fc961569f150
- Document Type :
- article
- Full Text :
- https://doi.org/10.1007/s40820-019-0257-1