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VERIFICATION OF THE MECHANISM OF GLYPHOSATE RESISTANCE IN ITALIAN RYEGRASS BIOTYPES

Authors :
L. VARGAS
Q. RUCHEL
D. AGOSTINETTO
F.P. LAMEGO
A.C. LANGARO
S.R. PIESANTI
Source :
Planta Daninha, Vol 34, Iss 3, Pp 565-573 (2016)
Publication Year :
2016
Publisher :
Sociedade Brasileira da Ciência das Plantas Daninhas, 2016.

Abstract

ABSTRACT The intense use of glyphosate for weed control led to the emergence of several cases of resistance to this herbicide. Weeds can survive the application of herbicides due to several factors, which may or may not be related to the herbicide site of action. The objectives of this study were to quantify the accumulation of shikimate in ryegrass biotypes in response to glyphosate application; investigate possible mutations on the EPSPs gene in susceptible and resistant biotypes; and evaluate the response of ryegrass biotypes to the application of glyphosate after treatment with a metabolism inhibitor of cyt P450 monooxygenase. The seeds of ryegrass biotypes with suspected resistance came from the municipality of São Valentim, RS (SVA 1 and SVA 4) and Passo Fundo, RS (PFU 5) and the seeds of the susceptible biotype (SVA 2), from São Valentim. The results demonstrated that, SVA biotype 2 accumulated more shikimate than any of the resistant biotypes, regardless of the herbicide dose used. The EPSPs gene showed no point mutation previously associated with the resistance to glyphosate, and the evaluated biotypes show no metabolism of glyphosate by the cyt P450 complex concerning inhibition by piperonyl butoxide (PBO) and malathion.

Details

Language :
English, Spanish; Castilian, Portuguese
ISSN :
01008358
Volume :
34
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Planta Daninha
Publication Type :
Academic Journal
Accession number :
edsdoj.3377d629210b48e1ad82f084bd2dce49
Document Type :
article
Full Text :
https://doi.org/10.1590/s0100-83582016340300017