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The Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation

Authors :
Paraskevi Prasinou
Ippolito De Amicis
Isa Fusaro
Roberta Bucci
Damiano Cavallini
Salvatore Parrillo
Maurizio Caputo
Alessandro Gramenzi
Augusto Carluccio
Source :
Animals, Vol 13, Iss 1, p 8 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Fatty acid-based lipidomic analysis has been widely used to evaluate health status in human medicine as well as in the veterinary field. In equine species, there has been a developing interest in fertility and sperm quality. Fatty acids, being the principal components of the membranes, play an active role in the regulation of the metabolic activities, and their role on spermiogenesis seems to be of great importance for the resulting quality of the sperm and, thus, fertility. With the application of widely used lipidomic techniques, the aim of this study was to evaluate: (a) the fatty acid content of the spermatozoa’s membranes of 26 healthy male Martina Franca donkeys and its possible correlation with sperm parameters, and (b) the evaluation of the composition of the red blood cells’ membrane. PUFA omega-6 are the principal components (40.38%) of the total PUFA content (47.79%) in both types of cells; however, DPA is the predominant one on the spermatozoa’s membrane (27.57%) but is not present in the erythrocyte’s membrane. Spermatozoa’s motility (%) is positively correlated with stearic acid and EPA, and progressive motility (%), with oleic acid. These findings offer information on the composition of both types of cells’ membranes in healthy male MF donkeys and reflect the metabolic transformations of the spermatozoa’s membrane during the maturation period, providing a better perception of the role of fatty acids in sperm parameters and fertility.

Details

Language :
English
ISSN :
20762615
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Animals
Publication Type :
Academic Journal
Accession number :
edsdoj.3343d3ffb349efa1064a7a1fd53816
Document Type :
article
Full Text :
https://doi.org/10.3390/ani13010008