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Essential role of CD38 in platelet aggregation through the PKC- mediated internalization and activation
- Source :
- BioImpacts, Vol 14, Iss 2, Pp 27780-27780 (2024)
- Publication Year :
- 2024
- Publisher :
- Tabriz University of Medical Sciences, 2024.
-
Abstract
- Introduction: CD38 is a multifunctional enzyme with a potent Ca2+ mobilizing effect, cyclic ADP-ribose (cADPR), and nicotinic acid adenine dinucleotide phosphate (NAADP). Here, we aimed to demonstrate the role of CD38 in platelets via protein kinase C (PKC)-mediated internalization and activation. Methods: Mouse platelets were used in this study. Thrombin, an agonist of platelet function, provoked a prompt and long-lasting increase in intracellular Ca2+ concentration ([Ca2+]i), resulting from an interplay of multifold Ca2+ mobilizing messengers.The signaling pathway was delineated using different inhibitors and techniques such as platelet aggregation assay, intracellular calcium measurements, immunoprecipitation, immunoblotting, and flow cytometry. Results: We observed a sequential formation of cADPR and NAADP through CD38 activation by PKC of non-muscle myosin heavy chain IIA (MHCIIA), resulting in phospholipase C (PLC) activation in the thrombin-stimulated platelets. These findings reveal that PKC is fundamental in activating CD38 and elicits a physiological response in the murine platelets. Conclusion: PKC is involved in many signaling pathways. Specifically, PKC is involved in the internalization of CD38 via MHCIIA in CD38+/+ wild-type (WT) and CD38-/- knockout mice (KO). CD38 generates calcium-mobilizing agents that act on specific receptors of the calcium stores. Calcium triggered platelet aggregation while serving as a secondary messenger.
Details
- Language :
- English
- ISSN :
- 22285652 and 22285660
- Volume :
- 14
- Issue :
- 2
- Database :
- Directory of Open Access Journals
- Journal :
- BioImpacts
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.322f2d1ba8a4c3d80bc519e92ec9456
- Document Type :
- article
- Full Text :
- https://doi.org/10.34172/bi.2023.27780