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Spread of ST348 Klebsiella pneumoniae Producing NDM-1 in a Peruvian Hospital

Authors :
Maria J. Pons
Marta Marí-Almirall
Barbara Ymaña
Jeel Moya-Salazar
Laura Muñoz
Sharon Sauñe
Richard Salazar-Hernández
Jordi Vila
Ignasi Roca
Source :
Microorganisms, Vol 8, Iss 9, p 1392 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae (CR-Kp) isolates recovered from adults and children with severe bacteremia in a Peruvian Hospital in June 2018. Antimicrobial susceptibility was determined by disc/gradient diffusion and broth microdilution when necessary. Antibiotic resistance mechanisms were evaluated by PCR and DNA sequencing. Clonal relatedness was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Plasmid typing was performed with a PCR-based method. Thirty CR-Kp isolates were recovered in June 2018. All isolates were non-susceptible to all β-lactams, ciprofloxacin, gentamicin and trimethoprim-sulfamethoxazole, while mostly remaining susceptible to colistin, tigecycline, levofloxacin and amikacin. All isolates carried the blaNDM-1 gene and were extended spectrum β-lactamase (ESBL) producers. PFGE showed four different pulsotypes although all isolates but two belonged to the ST348 sequence type, previously reported in Portugal. blaNDM-1 was located in an IncFIB-M conjugative plasmid. To our knowledge, this is the first report of an New Delhi metallo-β-lactamase (NDM)-producing K. pneumoniae recovered from both children and adults in Lima, Peru, as well as the first time that the outbreak strain ST348 is reported in Peru and is associated with NDM. Studies providing epidemiological and molecular data on CR-Kp in Peru are essential to monitor their dissemination and prevent further spread.

Details

Language :
English
ISSN :
20762607
Volume :
8
Issue :
9
Database :
Directory of Open Access Journals
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
edsdoj.3221720050741ff8e9cf1c7fe90c91e
Document Type :
article
Full Text :
https://doi.org/10.3390/microorganisms8091392