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Thermal Stabilisation of Lysozyme through Ensilication

Authors :
Reveng A. Abdulkareem
Aswin Doekhie
Nikoletta Fotaki
Francoise Koumanov
Charlotte A. Dodson
Asel Sartbaeva
Source :
Molecules, Vol 29, Iss 17, p 4207 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Protein therapeutics, vaccines, and other commercial products are often sensitive to environmental factors, such as temperature and long-term storage. In many cases, long-term protein stability is achieved by refrigeration or freezing. One alternative is the encapsulation of the protein cargo within an inert silica matrix (ensilication) and storage or transport at room temperature as a dry powder. In this paper, we test the effect of three commonly used biological buffers on the ensilication, storage, and desilication of the enzyme lysozyme. We show that ensilication protects lysozyme from heat (100 °C for 1 h) and during storage (18 months at room temperature). The choice of ensilication buffer has little effect on the activity of lysozyme after desilication. Our results provide confidence in the continued pursuit of ensilication as a methodology for protein stabilisation and in its compatibility with biological buffers.

Details

Language :
English
ISSN :
14203049
Volume :
29
Issue :
17
Database :
Directory of Open Access Journals
Journal :
Molecules
Publication Type :
Academic Journal
Accession number :
edsdoj.31720c39087649fa8c2630d59f027df1
Document Type :
article
Full Text :
https://doi.org/10.3390/molecules29174207