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T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses

Authors :
Ane Ogbe
Barbara Kronsteiner
Donal T. Skelly
Matthew Pace
Anthony Brown
Emily Adland
Kareena Adair
Hossain Delowar Akhter
Mohammad Ali
Serat-E Ali
Adrienn Angyal
M. Azim Ansari
Carolina V. Arancibia-Cárcamo
Helen Brown
Senthil Chinnakannan
Christopher Conlon
Catherine de Lara
Thushan de Silva
Christina Dold
Tao Dong
Timothy Donnison
David Eyre
Amy Flaxman
Helen Fletcher
Joshua Gardner
James T. Grist
Carl-Philipp Hackstein
Kanoot Jaruthamsophon
Katie Jeffery
Teresa Lambe
Lian Lee
Wenqin Li
Nicholas Lim
Philippa C. Matthews
Alexander J. Mentzer
Shona C. Moore
Dean J. Naisbitt
Monday Ogese
Graham Ogg
Peter Openshaw
Munir Pirmohamed
Andrew J. Pollard
Narayan Ramamurthy
Patpong Rongkard
Sarah Rowland-Jones
Oliver Sampson
Gavin Screaton
Alessandro Sette
Lizzie Stafford
Craig Thompson
Paul J. Thomson
Ryan Thwaites
Vinicius Vieira
Daniela Weiskopf
Panagiota Zacharopoulou
Oxford Immunology Network Covid-19 Response T Cell Consortium
Oxford Protective T Cell Immunology for COVID-19 (OPTIC) Clinical Team
Lance Turtle
Paul Klenerman
Philip Goulder
John Frater
Eleanor Barnes
Susanna Dunachie
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Understanding the immune response to SARS-CoV-2 is dependent on being able to distinguish COVID-19 immune responses from cross-reactive immune responses to other coronaviruses. Here the authors show that choice of antigens and whether an ICS, ELISPOT or T cell proliferation assay is used has a major effect on this discriminatory ability.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.316bcccee68f41c8b075256474e98d3e
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-21856-3