Dual drugs release from nanoporously bioactive coating on polyetheretherketone for enhancement of antibacterial activity, rBMSCs responses and osseointegration

A bioactive coating of nanoporous magnesium calcium silicate (n-MCS) on polyetheretherketone (ncPK) surface was prepared, and dual drugs of genistein (GS) and curcumin (CR) were co-loaded into coating of ncPK (dncPK). The results revealed that compared with polyetheretherketone (PEEK), surface roughness, wettability and apatite mineralization ability of both ncPK and dncPK obviously improved, which promoted the rat bone mesenchymal stem cells (rBMSCs) adhesion. In addition, compared with ncPK and PEEK, dncPK significantly promoted the proliferation and differentiation of rBMSCs. Compared with ncPK and PEEK, dncPK displayed superior antibacterial activity to Escherichia coli and Staphylococcus aureus. The Micro-CT, histological and push-out load evaluations revealed that compared with ncPK and PEEK, dncPK remarkably accelerated osteogenesis and osseointegration in vivo. The results demonstrated that dual drugs-loaded dncPK exhibited good antibacterial activity and osteogenic activity, and significantly enhanced osteogenesis and osseointegration, which could be an alternative candidate as an implant in orthopedic and dental applications. Keywords: Polyetheretherketone, Nanoporous coating, Dual drugs, Antibacterial activity, Osseointegration