Back to Search
Start Over
Benzo[a]pyrene degradation from hydrocarbon-contaminated soil and their degrading metabolites by Stutzerimonas stutzeri (LOBP-19A)
- Source :
- Waste Management Bulletin, Vol 1, Iss 3, Pp 115-127 (2023)
- Publication Year :
- 2023
- Publisher :
- Elsevier, 2023.
-
Abstract
- Polycyclic aromatic hydrocarbons (PAHs) are considered as harmful pollutants for the environment and human health system. The most prevalent PAHs in the environment is benzo[a]pyrene when present in high concentrations are potentially hazardous because it is carcinogenic. The objective of the current study was to identify and screen benzo[a]pyrene-degrading bacterial strain that is adapted for various environmental and nutritional requirements in order to determine how well they can decompose the benzo[a]pyrene. The results showed that, after 45 days of incubation at 140 rpm in a temperature-controlled shaker, benzo[a]pyrene (initial concentration of 1000 mg/L) was degraded 87.5% by the isolated strain LOBP-19A at 37 °C, pH 9 in the presence of glucose (1%), and peptone (0.5%) from. Isolated strain performed worse when temperature and pH are different from optimal conditions. Enzymes were screened using bacterial strain (LOBP-1A) and also produced biosurfactant by different method. GCMS studied and characterized metabolites of benzo[a]pyrene of LOBP-19A strain are 1-(3-methylbutyl)-2,3,4,6-tetramethylbenzene, Tetrazole, 1-(2-flourophenyl)-5-[4-(1,1-dimethylethyl) benzythio], 1,4-Benzenediol,2,6-bis(1,1-dimethylethyl)-(CAS), Octadecanoic acid trimethylsilyl ester and 1-naphthalene-sulfonic acid. The isolated strain was identified as Stutzerimonas stutzeri (LOBP-19A) OP389146 based their 16s-rRNA sequencing. Thus the current study demonstrated the great potential of isolated bacterial strain for use in water bodies and field scale bioremediation of benzo[a]pyrene and PAHs contaminated sites.
Details
- Language :
- English
- ISSN :
- 29497507
- Volume :
- 1
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Waste Management Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.30fc3d8d00754d62a6f97c709084bc1e
- Document Type :
- article
- Full Text :
- https://doi.org/10.1016/j.wmb.2023.07.006