Back to Search Start Over

De Novo Whole-Genome Sequencing and Assembly of the Yellow-Throated Bunting (Emberiza elegans) Provides Insights into Its Evolutionary Adaptation

Authors :
Tingli Hu
Guotao Chen
Zhen Xu
Site Luo
Hui Wang
Chunlin Li
Lei Shan
Baowei Zhang
Source :
Animals, Vol 12, Iss 15, p 2004 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Yellow-throated bunting is a small migratory songbird unique to the Palearctic region. However, the genetic studies of this species remain limited, with no nuclear genomic sequence reported to date. In this study, the genomic DNA from the bird was sequenced in long reads using Nanopore sequencing technology. Combining short-read sequencing, the genome was well-assembled and annotated. The final length of the assembly is approximately 1.14 Gb, with a scaffold N50 of 28.94 Mb. About 15,868 protein-coding genes were predicted, and 16.62% of the genome was identified as having repetitive elements. Comparative genomic analysis showed numerous expanded gene families and positively selected genes significantly enriched in those KEGG pathways that are associated with migratory behavior adaptation and immune response. Here, this newly generated de novo genome of the yellow-throated bunting using long reads provide the research community with a valuable resource for further studies of population genetic diversity and genome evolution in this species.

Details

Language :
English
ISSN :
20762615
Volume :
12
Issue :
15
Database :
Directory of Open Access Journals
Journal :
Animals
Publication Type :
Academic Journal
Accession number :
edsdoj.30279e75f6fb4f1ca7430cb74cd0191f
Document Type :
article
Full Text :
https://doi.org/10.3390/ani12152004