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Evaluation of the potential food allergy risks of human lactoferrin expressed in Komagataella phaffii

Authors :
Yanisa Anaya
Raysa Rosario Martinez
Richard E. Goodman
Philip Johnson
Shashwat Vajpeyi
Xiaoning Lu
Ross Peterson
Sarah M. Weyers
Bella Breen
Kahler Newsham
Brian Scottoline
Anthony J. Clark
Carrie-Anne Malinczak
Source :
Frontiers in Immunology, Vol 15 (2024)
Publication Year :
2024
Publisher :
Frontiers Media S.A., 2024.

Abstract

IntroductionPrior to the introduction of novel food ingredients into the food supply, safety risk assessments are required, and numerous prediction models have been developed and validated to evaluate safety.MethodsThe allergenic risk potential of Helaina recombinant human lactoferrin (rhLF, Efferaâ„¢), produced in Komagataella phaffii (K. phaffii) was assessed by literature search, bioinformatics sequence comparisons to known allergens, glycan allergenicity assessment, and a simulated pepsin digestion model.ResultsThe literature search identified no allergenic risk for Helaina rhLF, K. phaffii, or its glycans. Bioinformatics search strategies showed no significant risk for cross-reactivity or allergenicity between rhLF or the 36 residual host proteins and known human allergens. Helaina rhLF was also rapidly digested in simulated gastric fluid and its digestibility profile was comparable to human milk lactoferrin (hmLF), further demonstrating a low allergenic risk and similarity to the hmLF protein. ConclusionCollectively, these results demonstrate a low allergenic risk potential of Helaina rhLF and do not indicate the need for further clinical testing or serum IgE binding to evaluate Helaina rhLF for risk of food allergy prior to introduction into the food supply.

Details

Language :
English
ISSN :
16643224
Volume :
15
Database :
Directory of Open Access Journals
Journal :
Frontiers in Immunology
Publication Type :
Academic Journal
Accession number :
edsdoj.301dcddd3e6c42e09a9e5e13adec11b1
Document Type :
article
Full Text :
https://doi.org/10.3389/fimmu.2024.1380028