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13C Isotope Labelling to Follow the Flux of Photorespiratory Intermediates

Authors :
Cyril Abadie
Guillaume Tcherkez
Source :
Plants, Vol 10, Iss 3, p 427 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Measuring the carbon flux through metabolic pathways in intact illuminated leaves remains challenging because of, e.g., isotopic dilution by endogenous metabolites, the impossibility to reach isotopic steady state, and the occurrence of multiple pools. In the case of photorespiratory intermediates, our knowledge of the partitioning between photorespiratory recycling, storage, and utilization by other pathways is thus rather limited. There has been some controversy as to whether photorespiratory glycine and serine may not be recycled, thus changing the apparent stoichiometric coefficient between photorespiratory O2 fixation and CO2 release. We describe here an isotopic method to trace the fates of glycine, serine and glycerate, taking advantage of positional 13C content with NMR and isotopic analyses by LC–MS. This technique is well-adapted to show that the proportion of glycerate, serine and glycine molecules escaping photorespiratory recycling is very small.

Details

Language :
English
ISSN :
22237747
Volume :
10
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Plants
Publication Type :
Academic Journal
Accession number :
edsdoj.2ffc72c8c8c49d3aac980e11792c973
Document Type :
article
Full Text :
https://doi.org/10.3390/plants10030427