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Analysis of Osteosarcoma Cell Lines and Patient Tissue Using a 3D In Vivo Tumor Model—Possible Effects of Punicalagin

Authors :
Anna Rebecca Dorn
Sara Neff
Sophia Hupp
Melissa Engelhardt
Eric Pion
Ulrich Lenze
Carolin Knebel
Anna Duprée
Simone Schewe
Markus Weber
Christian Wulbrand
Axel Hillmann
Florian Weber
Phillip Clarke
Philipp Kainz
Thiha Aung
Silke Haerteis
Source :
Organoids, Vol 3, Iss 1, Pp 35-53 (2024)
Publication Year :
2024
Publisher :
MDPI AG, 2024.

Abstract

Osteosarcomas are the most common primary malignant bone tumors and mostly affect children, adolescents, and young adults. Despite current treatment options such as surgery and polychemotherapy, the survival of patients with metastatic disease remains poor. In recent studies, punicalagin has reduced the cell viability, angiogenesis, and invasion in cell culture trials. The aim of this study was to examine the effects of punicalagin on osteosarcomas in a 3D in vivo tumor model. Human osteosarcoma biopsies and SaOs-2 and MG-63 cells, were grown in a 3D in vivo chorioallantoic membrane (CAM) model. After a cultivation period of up to 72 h, the tumors received daily treatment with punicalagin for 4 days. Weight measurements of the CAM tumors were performed, and laser speckle contrast imaging (LSCI) and a deep learning-based image analysis software (CAM Assay Application v.3.1.0) were used to measure angiogenesis. HE, Ki-67, and Caspase-3 staining was performed after explantation. The osteosarcoma cell lines SaOs-2 and MG-63 and osteosarcoma patient tissue displayed satisfactory growth patterns on the CAM. Treatment with punicalagin decreased tumor weight, proliferation, and tumor-induced angiogenesis, and the tumor tissue showed pro-apoptotic characteristics. These results provide a robust foundation for the implementation of further studies and show that punicalagin offers a promising supplementary treatment option for osteosarcoma patients. The 3D in vivo tumor model represents a beneficial model for the testing of anti-cancer therapies.

Details

Language :
English
ISSN :
26741172
Volume :
3
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Organoids
Publication Type :
Academic Journal
Accession number :
edsdoj.2f3ce05ca38c4906b406f1d7b6bf04fe
Document Type :
article
Full Text :
https://doi.org/10.3390/organoids3010004