Association Analyses of SNAP25, HNMT, FCHSD1, and DBH Single-Nucleotide Polymorphisms with Parkinson’s Disease in a Northern Chinese Population

Cuiyun Dai, Yichi Zhang, Xiaoni Zhan, Meihui Tian, Hao Pang Department of Forensic Genetics and Biology, School of Forensic Medicine, China Medical University, Shenyang, 110122, People’s Republic of ChinaCorrespondence: Hao Pang Tel +86-24-31939435Email hpang@cmu.edu.cnPurpose: Sequencing potentially causal and susceptible genes and genome-wide association studies in samples from Parkinson’s disease (PD) patients has revealed several related loci. The genes for synaptosome-associated protein of 25 kDa (SNAP25), histamine-N-methyltransferase (HNMT), FCH and double SH3 domains 1 (FCHSD1) and dopamine β-hydroxylase (DBH) are candidate loci and have not been studied in a northern Chinese population. We explored the genetic distribution of four single-nucleotide polymorphisms (rs3746544, rs11558538, rs456998, rs129882) located on SNAP25, HNMT, FCHSD1 and DBH, respectively.Patients and Methods: A total of 330 patients with sporadic PD and 332 healthy controls (HCs) were recruited from a northern Chinese population. Polymerase chain reaction restriction fragment length polymorphism was used to genotype these four SNPs.Results: After statistical analyses and correction of the genotyping results, the mutant-allele T in rs456998 of FCHSD1 was found to be significantly related to reducing the PD risk (P = 0.029, OR = 0.754, 95% CI = 0.586– 0.971, power = 0.591). However, rs3746544, rs11558538, and rs129882 did not show an association with PD.Conclusion: FCHSD1 rs456998 may have a protective role in PD in a northern Chinese population, but more studies are needed to support this suggestion.Keywords: Parkinson’s disease, single nucleotide polymorphism, SNAP25, HNMT, FCHSD1, DBH, association study