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Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity

Authors :
Frédéric Doussau
Hartmut Schmidt
Kevin Dorgans
Antoine M Valera
Bernard Poulain
Philippe Isope
Source :
eLife, Vol 6 (2017)
Publication Year :
2017
Publisher :
eLife Sciences Publications Ltd, 2017.

Abstract

The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum.

Details

Language :
English
ISSN :
2050084X
Volume :
6
Database :
Directory of Open Access Journals
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
edsdoj.2db80c8d7a4ec4870543bff33b7f83
Document Type :
article
Full Text :
https://doi.org/10.7554/eLife.28935