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A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses

Authors :
Alberto A. Amarilla
Julian D. J. Sng
Rhys Parry
Joshua M. Deerain
James R. Potter
Yin Xiang Setoh
Daniel J. Rawle
Thuy T. Le
Naphak Modhiran
Xiaohui Wang
Nias Y. G. Peng
Francisco J. Torres
Alyssa Pyke
Jessica J. Harrison
Morgan E. Freney
Benjamin Liang
Christopher L. D. McMillan
Stacey T. M. Cheung
Darwin J. Da Costa Guevara
Joshua M. Hardy
Mark Bettington
David A. Muller
Fasséli Coulibaly
Frederick Moore
Roy A. Hall
Paul R. Young
Jason M. Mackenzie
Jody Hobson-Peters
Andreas Suhrbier
Daniel Watterson
Alexander A. Khromykh
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.2d42390e6b6242feac70593cf3d675b1
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-021-23779-5