Construction of sterile system of Buchloe dactyloides (Nutt.) Engelm.

The stems of male and female strains of Bison grass (Buchloe dactyloides (Nutt.) Engelm.) were taken as explants, and 1 / 2MS and MS medium were used, and different concentrations of NAA, KT and 6-BA were added to study the rapid propagation technology of ferison grass. Results indicated that 75% alcohol was treated for 30s with another 0.1 g ꞏL-1The disinfection effect of mercury solution for 12 min; The MS + 1.5 mg ꞏL-1 NAA + 0.4 mg ꞏL-1 KT female strain showed the best induction effect, The MS + 1.5 mg ꞏL-1 NAA + 0.6 mg ꞏL-1 KT male strain had the best induction effect; 1 / 2 MS + 2 mg + L-1 NAA + 1.5 mg ꞏL-1 6-BA the female and male strains had the best proliferation effect; The highest number of roots and root root rate of 1 / 2 MS + 1.5 mg ꞏL-1 female and male NAA strains were combined.