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Optimization of Accelerated Solvent Extraction of Polysaccharides from Gracilaria lemaneiformis Using Response Surface Methodology and Anti-inflammatory Activity
- Source :
- Shipin gongye ke-ji, Vol 44, Iss 23, Pp 110-117 (2023)
- Publication Year :
- 2023
- Publisher :
- The editorial department of Science and Technology of Food Industry, 2023.
-
Abstract
- The aim of this work was to apply response surface methodology (RSM) to model and optimize the accelerated solvent extraction (ASE) technique for extracting the crude polysaccharides (GLP-K) from Gracilaria lemaneiformis. In terms of the yield of polysaccharide, single factor tests and Box-Behnken design response surface method were developed. The structure of the prepared polysaccharides was characterized by Fourier transform infrared (FT-IR) and high performance liquid chromatography (HPLC). And the anti-inflammatory potential of GLP-K in lipopolysaccharide (LPS)-induced RAW264.7 macrophages was also explored. Briefly, the optimal extraction conditions for GLP-K were as follows: 70 °C of extraction temperature, 8.5 min of extraction time, and 4 extraction cycles. Under the condition, the experimental yield of polysaccharide was 9.58%±0.31%. FT-IR showed that the polysaccharide contained uronic acid, and the weight-average molecular weight ranged from 4.4 to 747.1 kDa. GLP-K had no significant cytotoxic effects at or below the concentrations of 1000 μg/mL (P
Details
- Language :
- Chinese
- ISSN :
- 10020306
- Volume :
- 44
- Issue :
- 23
- Database :
- Directory of Open Access Journals
- Journal :
- Shipin gongye ke-ji
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.2a583784047d0bd42918fd185b3d2
- Document Type :
- article
- Full Text :
- https://doi.org/10.13386/j.issn1002-0306.2023030309