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Rapamycin protects testes against germ cell apoptosis and oxidative stress induced by testicular ischemia-reperfusion

Authors :
Morteza Ghasemnejad-berenji
Mahmoud Ghazi-Khansari
Iraj Yazdani
Seyed Soheil Saeedi Saravi
Maliheh Nobakht
Alireza Abdollahi
Javad Mohajer Ansari
Hojjat Ghasemnejad-berenji
Sarvin Pashapour
Ahmad Reza Dehpour
Source :
Iranian Journal of Basic Medical Sciences, Vol 20, Iss 8, Pp 905-911 (2017)
Publication Year :
2017
Publisher :
Mashhad University of Medical Sciences, 2017.

Abstract

Objective(s):Rapamycin is an immunosuppressant compound with a broad spectrum of pharmaco-logical activities. In recent years, it has been used successfully to decrease ischemia-reperfusion injury in several organ systems. The purpose of the present study was to examine the effect of rapamycin on testicular ischemia-reperfusion injury. Materials and Methods: Seventy-two adult male Wistar rats were divided into six groups: control (group1), sham-operated (Group2), T/D + DMSO as vehicle group (group3), and groups 4–6; respectively received 0.5, 1, and 1.5 mgkg-1 of rapamycin , IP 30 min before detorsion. Ischemia was achieved by twisting the right testis 720o clockwise for 1 hr. The right testis of 6 animals from each group were excised 4 hr after detorsion for the measurement of lipid peroxidation, caspase-3, and antioxidant enzyme activities. Histopathological changes and germ cell apoptosis were determined by measuring mean of seminiferous tubules diameters (MSTD) and TUNEL test in right testis of 6 animals per group, 24 hr after detorsion. Results: Testicular T/D caused increases in the apoptosis, malondialdehyde (MDA), and caspase-3 levels and decreases in the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities in ipsilateral testis (P

Details

Language :
English
ISSN :
20083866 and 20083874
Volume :
20
Issue :
8
Database :
Directory of Open Access Journals
Journal :
Iranian Journal of Basic Medical Sciences
Publication Type :
Academic Journal
Accession number :
edsdoj.29ea9ef64b7746269079913c3ab905b1
Document Type :
article
Full Text :
https://doi.org/10.22038/ijbms.2017.9112