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pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4CRBN ubiquitin ligase

Authors :
Jian An
Charles M. Ponthier
Ragna Sack
Jan Seebacher
Michael B. Stadler
Katherine A. Donovan
Eric S. Fischer
Source :
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Publication Year :
2017
Publisher :
Nature Portfolio, 2017.

Abstract

Targeting therapeutically-relevant proteins for degradation is an emerging paradigm in drug discovery. Here the authors describe a sensitive pulse SILAC mass spectrometry-based proteomics approach that reports global changes in protein stability following drug treatment in a single time point experiment.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
8
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.29e7aa9bcbbf457cab317eff9b4c1bbd
Document Type :
article
Full Text :
https://doi.org/10.1038/ncomms15398