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Polyphenol analysis in human milk by a rapid, cost-effective, and accurate micromethod: translational development
- Source :
- Revista Paulista de Pediatria, Vol 42 (2024)
- Publication Year :
- 2024
- Publisher :
- Sociedade de Pediatria de São Paulo, 2024.
-
Abstract
- ABSTRACT Objective: To develop a rapid method for analysing polyphenols, which are potentially active antioxidants against neonatal oxidative stress, from small human milk (HM) volumes. Methods: Acid and alkaline extractions were compared using two dyes: Folin-Ciocalteu and Fast Blue BB. Linearity, sensitivity, recovery percentage, polyphenol content, precision, and stability were assessed in 14 HM samples and compared using the Kruskal-Wallis H test (p0.96) up to 500 mg/L. All bicarbonate-based Folin-Ciocalteu methods assayed were stable and repeatable, whereas Fast Blue BB-based variants were not. HM polyphenols (mean=94.68 mg/L) positively correlated to the dietary intake of hydroxycinnamic acids, the most consumed polyphenolic family in this population. Conclusions: A bicarbonate-based Folin-Ciocalteu micromethod allowed the accurate determination of polyphenols in HM, which might be useful for translational research settings and HM banks.
Details
- Language :
- English, Spanish; Castilian, Portuguese
- ISSN :
- 19840462
- Volume :
- 42
- Database :
- Directory of Open Access Journals
- Journal :
- Revista Paulista de Pediatria
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.28c86dcf9ae041e68962e900fb8d3546
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/1984-0462/2024/42/2023186