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The effect of age on the microarchitecture and profile of gene expression in femoral head and neck bone from patients with osteoarthritis

Authors :
Dorit Naot
Maureen Watson
Ally J. Choi
David S. Musson
Karen E. Callon
Mark Zhu
Ryan Gao
William Caughey
Rocco P. Pitto
Jacob T. Munro
Anne Horne
Gregory D. Gamble
Nicola Dalbeth
Ian R. Reid
Jillian Cornish
Source :
Bone Reports, Vol 13, Iss , Pp 100287- (2020)
Publication Year :
2020
Publisher :
Elsevier, 2020.

Abstract

Ageing of the skeleton is characterised by decreased bone mineral density, reduced strength, and increased risk of fracture. Although it is known that these changes are determined by the activities of bone cells through the processes of bone modelling and remodelling, details of the molecular mechanisms that underlie age-related changes in bone are still missing. Here, we analysed age-related changes in bone microarchitecture along with global gene expression in samples obtained from patients with osteoarthritis (OA). We hypothesised that changes would be evident in both microarchitecture and gene expression and aimed to identify novel molecular mechanisms that underlie ageing processes in bone. Samples of femoral head and neck were obtained from patients undergoing hip arthroplasty for OA, who were either ≤60 years or ≥70 years of age. Bone microarchitecture was analysed in cores of trabecular bone from the femoral head (17 from the younger group and 18 from the older), and cortical bone from the femoral neck (25 younger/22 older), using a Skyscan 1172 microCT scanner (Bruker). Gene expression was compared between the two age groups in 20 trabecular samples from each group, and 10 cortical samples from each group, using Clariom S Human microarrays (ThermoFisher Scientific). We found no significant changes between the two age groups in indices of trabecular or cortical bone microarchitecture. Gene expression analysis identified seven genes that had higher expression in the older group, including the transcription factor EGR1 and the glucose transporter SLC2A3 (GLUT3), and 21 differentially expressed genes in cortical bone samples (P2). However, none of the comparisons of gene expression had false discovery rate-adjusted P

Details

Language :
English
ISSN :
23521872
Volume :
13
Issue :
100287-
Database :
Directory of Open Access Journals
Journal :
Bone Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.28808ad50c2d442e99d2f38beb80fa0c
Document Type :
article
Full Text :
https://doi.org/10.1016/j.bonr.2020.100287