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Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes
- Source :
- Microbial Cell, Vol 6, Iss 1, Pp 65-101 (2019)
- Publication Year :
- 2019
- Publisher :
- Shared Science Publishers OG, 2019.
-
Abstract
- Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
- Subjects :
- chromatin dynamics
chromosome rearrangements
crossovers
DNA breaks
DNA helicases
DNA repair centers
DNA repair synthesis
DNA resection
double strand break repair
DSBs
endonuclease protection assay
genome instability
gross chromosome rearrangements
fluorescent proteins
FRET
homologous recombination
mismatch repair
nonhomologous end joining
nucleotide excision repair
PALM
photoactivated fluorescent proteins
recombinase filament assembly
single-molecule
single-particle tracking
super resolution
structure-selective endonucleases
synthesis-dependent strand annealing
transcription coupled repair
Biology (General)
QH301-705.5
Subjects
Details
- Language :
- English
- ISSN :
- 23112638
- Volume :
- 6
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Microbial Cell
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.284d1fa54b1b42a89ebab92b52616ba5
- Document Type :
- article
- Full Text :
- https://doi.org/10.15698/mic2019.01.665