Back to Search Start Over

Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model

Authors :
Amanda Mara Teles
Leticia Prince Pereira Pontes
Sulayne Janayna Araújo Guimarães
Ana Luiza Butarelli
Gabriel Xavier Silva
Flavia Raquel Fernandes do Nascimento
Geusa Felipa de Barros Bezerra
Carla Junqueira Moragas-Tellis
Rui Miguel Gil da Costa
Marcos Antonio Custódio Neto da Silva
Fernando Almeida-Souza
Kátia da Silva Calabrese
Ana Paula Silva Azevedo-Santos
Maria do Desterro Soares Brandão Nascimento
Source :
Marine Drugs, Vol 18, Iss 11, p 541 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Background: This study addresses the antitumoral properties of Penicillium purpurogenum isolated from a polluted lagoon in Northeastern Brazil. Methods: Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups (n = 10/group) as follows: The negative control (CTL−), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. Results: The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. Conclusions: These results indicate that P. purpurogenum exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents.

Details

Language :
English
ISSN :
18110541 and 16603397
Volume :
18
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Marine Drugs
Publication Type :
Academic Journal
Accession number :
edsdoj.27dbdd22c08e491da818ebab4ddfa00a
Document Type :
article
Full Text :
https://doi.org/10.3390/md18110541