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Background Noise Suppression of Optical Sectioning Structured Illumination Microscopy via Fourier Domain Reconstruction

Authors :
Shipei Dang
Jia Qian
Tong Peng
Chen Bai
Junwei Min
Haixia Wang
Baoli Yao
Dan Dan
Source :
Frontiers in Physics, Vol 10 (2022)
Publication Year :
2022
Publisher :
Frontiers Media S.A., 2022.

Abstract

Optical sectioning structured illumination microscopy (OS-SIM) has been attracting considerable interest in fast 3D microscopy. The reconstruction of optical sectioning images in the conventional method employs the root-mean-square (RMS) algorithm in the spatial domain, which is prone to residual background noise. To overcome this problem, we propose a Fourier domain based optical sectioning image reconstruction algorithm (termed Fourier-OS-SIM), which has an improved background noise suppression capability compared to the RMS algorithm. The experimental results verified the feasibility and the effectiveness of the algorithm. The improved performance of the Fourier-OS-SIM may find more applications in biomedical or industrial fields.

Details

Language :
English
ISSN :
2296424X
Volume :
10
Database :
Directory of Open Access Journals
Journal :
Frontiers in Physics
Publication Type :
Academic Journal
Accession number :
edsdoj.27b01af316ac4eba96b8cc5b03b94b58
Document Type :
article
Full Text :
https://doi.org/10.3389/fphy.2022.900686