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Antimicrobial Susceptibility and Characterization of Resistance Mechanisms of Corynebacterium urealyticum Clinical Isolates

Authors :
Itziar Chapartegui-González
Marta Fernández-Martínez
Ana Rodríguez-Fernández
Danilo J. P. Rocha
Eric R. G. R. Aguiar
Luis G. C. Pacheco
José Ramos-Vivas
Jorge Calvo
Luis Martínez-Martínez
Jesús Navas
Source :
Antibiotics, Vol 9, Iss 7, p 404 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterial, most frequently involved in urinary tract infections. Most of the C. urealyticum clinical isolates are frequently resistant to several antibiotics. We investigated the susceptibility of 40 C. urealyticum isolated in our institution during the period 2005–2017 to eight compounds representative of the main clinically relevant classes of antimicrobial agents. Antimicrobial susceptibility was determined by the Epsilometer test. Resistance genes were searched by PCR. All strains were susceptible to vancomycin whereas linezolid and rifampicin also showed good activity (MICs90 = 1 and 0.4 mg/L, respectively). Almost all isolates (39/40, 97.5%) were multidrug resistant. The highest resistance rate was observed for ampicillin (100%), followed by erythromycin (95%) and levofloxacin (95%). Ampicillin resistance was associated with the presence of the blaA gene, encoding a class A β-lactamase. The two rifampicin-resistant strains showed point mutations driving amino acid replacements in conserved residues of RNA polymerase subunit β (RpoB). Tetracycline resistance was due to an efflux-mediated mechanism. Thirty-nine PFGE patterns were identified among the 40 C. urealyticum, indicating that they were not clonally related, but producing sporadic infections. These findings raise the need of maintaining surveillance strategies among this multidrug resistant pathogen.

Details

Language :
English
ISSN :
20796382
Volume :
9
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Antibiotics
Publication Type :
Academic Journal
Accession number :
edsdoj.26fcff19d4ff4e0fafdcb2808306cc4e
Document Type :
article
Full Text :
https://doi.org/10.3390/antibiotics9070404