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Multidrug-resistant and enterotoxigenic methicillin-resistant Staphylococcus aureus isolated from raw milk of cows at small-scale production units

Authors :
Hamza Mohamed Eid
Heba Sayed El-Mahallawy
Sahar Roshdi Mohammed
Nehal Ezzat Yousef Mohammed
Nada Hussein Eidaroos
Source :
Journal of Advanced Veterinary and Animal Research, Vol 9, Iss 1, Pp 113-121 (2022)
Publication Year :
2022
Publisher :
Network for the Veterinarians of Bangladesh, 2022.

Abstract

Objective: Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate the prevalence, antibiotic sensitivity, virulence, and enterotoxin production of S. aureus in raw milk of cow from small-scale production units and house-raised animals in Damietta governorate, Egypt. Material and Methods: The samples were examined bacteriologically, and antimicrobial sensitivity testing was carried out. Moreover, isolates were characterized by the molecular detection of antimicrobial resistance, virulence, and enterotoxin genes. Results: Out of 300 milk samples examined, S. aureus was isolated from 50 samples (16.7%). Antibiotic sensitivity testing revealed that isolates were resistant to β-lactams (32%), tetracycline (16%), and norfloxacin (16%); however, they showed considerable sensitivity to ceftaroline and amikacin (72%). Multidrug-resistance (MDR) has been observed in eight isolates (16%), with a MDR index (0.5) in all of them. Of the total S. aureus isolates obtained, methicillin-resistant S. aureus (MRSA) has been confirmed molecularly in 16/50 (32%) and was found to carry mecA and coa genes, while virulence genes; hlg (11/16, 68.75%) and tsst (6/16, 37.5%) were amplified at a lower percentage, and they showed a significant moderate negative correlation (r = −0.59, p-value > 0.05). Antibiotic resistance genes have been detected in resistant isolates relevant to their phenotypic resistance: blaZ (100%), tetK (50%), and norA (50%). Fifty percent of MRSA isolates carried the seb enterotoxin gene. Conclusion: High detection rate of MRSA and MDR isolates from milk necessitates the prompt implementation of efficient antimicrobial stewardship guidelines, especially at neglected small-scale production units. [J Adv Vet Anim Res 2022; 9(1.000): 113-121]

Details

Language :
English
ISSN :
23117710
Volume :
9
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Journal of Advanced Veterinary and Animal Research
Publication Type :
Academic Journal
Accession number :
edsdoj.26d435b9e3d8429291b362af70546a03
Document Type :
article
Full Text :
https://doi.org/10.5455/javar.2022.i575