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Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
- Source :
- Química Nova, Vol 31, Iss 3, Pp 574-578 (2008)
- Publication Year :
- 2008
- Publisher :
- Sociedade Brasileira de Química, 2008.
-
Abstract
- A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.
- Subjects :
- etoricoxib
LC-APCI/MS/MS
on-line solid-phase extraction
Chemistry
QD1-999
Subjects
Details
- Language :
- English, Spanish; Castilian, Portuguese
- ISSN :
- 01004042 and 16787064
- Volume :
- 31
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Química Nova
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.26d2c084b6a4435e8f789b10590f6742
- Document Type :
- article
- Full Text :
- https://doi.org/10.1590/S0100-40422008000300021