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An electronic circular dichroism spectroscopy method for the quantification of L- and D-amino acids in enantiomeric mixtures
- Source :
- Royal Society Open Science, Vol 8, Iss 3 (2021)
- Publication Year :
- 2021
- Publisher :
- The Royal Society, 2021.
-
Abstract
- Rapid quantitative analysis of single chiral amino acid (aa) was achieved using circular dichroism (CD) with data analysis by standard calibration curve. The absolute concentrations of D- and L-aas in enantiomeric mixtures were determined by CD and achiral liquid chromatography (LC) method. It is worth noting that CD and LC were used independently, not online LC/CD in this study. The errors of the experimental results were less than 10%. The method is also applicable to the quantification of non-aa chiral molecules, such as chiral nucleoside and chiral quinine. With this study, we provide a new method for the chiral quantitative analysis of enantiomeric aas mixtures.
- Subjects :
- circular dichroism
amino acid
enantiomer
quantification
Science
Subjects
Details
- Language :
- English
- ISSN :
- 20545703
- Volume :
- 8
- Issue :
- 3
- Database :
- Directory of Open Access Journals
- Journal :
- Royal Society Open Science
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.260df92efae14411a171648da5a5e32b
- Document Type :
- article
- Full Text :
- https://doi.org/10.1098/rsos.201963