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Bioeffects of silver nanoparticles (AgNPs) synthesized by producer of biosurfactant Bacillus subtilis strain: in vitro cytotoxicity, antioxidant properties and metabolic activities of mammalian cells
- Source :
- Archives of Environmental Protection, Vol vol. 48, Iss No 4, Pp 45-52 (2022)
- Publication Year :
- 2022
- Publisher :
- Polish Academy of Sciences, 2022.
-
Abstract
- The present study is focused on the evaluation of bioeffects of silver nanoparticles (AgNPs) synthesized by Bacillus subtilis strain I’-1a, the producer of iturin A lipopeptide biosurfactant. The following properties of biologically synthesized silver nanoparticles (bio-AgNPs) were evaluated: in vitro cytotoxicity, antioxidant properties, and metabolic activities of mammalian cells. As a control, chemically synthesized silver nanoparticles (chem-AgNPs) were used. In vitro, antioxidant activity of bio-AgNPs showed a significant effect on the scavenging of free radicals. Bio-AgNPs can be potent natural antioxidants and can be essential for health preservation against oxidative stress-related degenerative diseases, such as cancer. The cell viability of human skin fibroblasts NHDF was remarkably inhibited in the presence of both AgNPs. However, bio-AgNPs were more active than chem-AgNPs. In our experiment, microarrays PM-M1–PM-M4 were used to evaluate the growth of NHDF fibroblast cells in the presence of bio-AgNPs and chem-AgNPs. The NHDF fibroblast cells were more active in the presence of bio-AgNPs than in chem-AgNPs. Probably, the presence of biosurfactant produced by Bacillus subtilis I’-1a significantly increased the stability of biogenic AgNPs and enhanced their biological activities and specific interaction with human DNA. Furthermore, the evaluated biological activities were enhanced for the biosurfactant-based AgNPs.
Details
- Language :
- English
- ISSN :
- 20834772 and 20834810
- Volume :
- . 48
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Archives of Environmental Protection
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.25bc1fc41bf4425387ab8148df3f9ddc
- Document Type :
- article
- Full Text :
- https://doi.org/10.24425/aep.2022.143708