A simplified LC−MS/MS approach for simultaneous quantification and pharmacokinetics of five compounds in rats following oral administration of Gastrodia elata extract

Abstract Background To support the multicomponent pharmacokinetics of Gastrodia elata, a rapid, simple, and sensitive ultra-performance liquid chromatography tandem with mass spectrometry (LC–MS/MS) approach was established for simultaneous quantification of gastrodin, parishin A, parishin B, parishin C, and parishin E. Methods Five compounds were extracted from plasma by using one-step protein precipitation. The chromatographic separation was achieved on a C18 column with gradient mobile phase comprising acetonitrile and 0.05% formic acid. The detection was performed using negative electrospray ionization in multiple reaction monitoring mode. Results This new method maximizes assay throughput by using minimal sample clean-up procedures and a shorter analytical run time. The approach exhibited good linearity for the five compounds (r 2 > 0.995) in the concentration ranges. The lower limits of quantification (LLOQ) were determined as 1.37 ng/mL for parishin A, parishin B, parishin C, and parishin E and 10 ng/mL for gastrodin. Then the method was fully validated with intra- and inter-day precision, accuracy, matrix effects, extraction recovery, and stability. Conclusion This validated approach was successfully applied to the pharmacokinetic study following oral administration of Gastrodia elata extract to rats. This investigation may provide some guidance for the clinical application and explanation of the pharmacological mechanism of Gastrodia elata. Graphical abstract