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Functional Oocytes Derived from Granulosa Cells

Functional Oocytes Derived from Granulosa Cells

Authors :
Chenglei Tian
Linlin Liu
Xiaoying Ye
Haifeng Fu
Xiaoyan Sheng
Lingling Wang
Huasong Wang
Dai Heng
Lin Liu
Source :
Cell Reports, Vol 29, Iss 13, Pp 4256-4267.e9 (2019)
Publication Year :
2019
Publisher :
Elsevier, 2019.

Abstract

Summary: The generation of genomically stable and functional oocytes has great potential for preserving fertility and restoring ovarian function. It remains elusive whether functional oocytes can be generated from adult female somatic cells through reprogramming to germline-competent pluripotent stem cells (gPSCs) by chemical treatment alone. Here, we show that somatic granulosa cells isolated from adult mouse ovaries can be robustly induced to generate gPSCs by a purely chemical approach, with additional Rock inhibition and critical reprogramming facilitated by crotonic sodium or acid. These gPSCs acquired high germline competency and could consistently be directed to differentiate into primordial-germ-cell-like cells and form functional oocytes that produce fertile mice. Moreover, gPSCs promoted by crotonylation and the derived germ cells exhibited longer telomeres and high genomic stability like PGCs in vivo, providing additional evidence supporting the safety and effectiveness of chemical induction, which is particularly important for germ cells in genetic inheritance. : Tian et al. report the successful generation of functional oocytes with genomic stability that produce fertile pups from adult-granulosa-cell-derived gPSCs through chemical reprogramming, which exhibits great potential for preserving fertility and restoring ovarian function. Keywords: chemical reprogramming, pluripotent stem cell, oocyte, granulosa cell

Subjects

Subjects :
Biology (General)
QH301-705.5

Details

Language :
English
ISSN :
22111247
Volume :
29
Issue :
13
Database :
Directory of Open Access Journals
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
edsdoj.24859385e97b4ae6b51ac33a91ca3b5d
Document Type :
article
Full Text :
https://doi.org/10.1016/j.celrep.2019.11.080