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Identification of a cDNA from the Arbuscular Mycorrhizal Fungus Glomus intraradices that is Expressed During Mycorrhizal Symbiosis and Up-Regulated by N Fertilization
- Source :
- Molecular Plant-Microbe Interactions, Vol 15, Iss 4, Pp 360-367 (2002)
- Publication Year :
- 2002
- Publisher :
- The American Phytopathological Society, 2002.
-
Abstract
- A cDNA library was constructed with RNA from Glomus intraradices-colonized lettuce roots and used for differential screening. This allowed the identification of a cDNA (Gi-1) that was expressed only in mycorrhizal roots and was of fungal origin. The function of the gene product is unknown, because Gi-1 contained a complete open reading frame that was predicted to encode a protein of 157 amino acids which only showed little homology with glutamine synthetase from Helicobacter pylori. The time-course analysis of gene expression during the fungal life cycle showed that Gi-1 was expressed only during the mycorrhizal symbiosis and was not detected in dormant or germinating spores of G. intraradices. P fertilization did not significantly change the pattern of Gi-1 expression compared with that in the unfertilized treatment, whereas N fertilization (alone or in combination with P) considerably enhanced the Gi-1 transcript accumulation. This increase in gene expression correlated with plant N status and growth under such conditions. The possible role of the Gi-1 gene product in intermediary N metabolism of arbuscular mycorrhizal symbiosis is further discussed.
- Subjects :
- AM symbiosis
drought stress
N metabolism
Microbiology
QR1-502
Botany
QK1-989
Subjects
Details
- Language :
- English
- ISSN :
- 19437706 and 08940282
- Volume :
- 15
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- Molecular Plant-Microbe Interactions
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.22c3ffeba29a455d91c5777eb8d2c553
- Document Type :
- article
- Full Text :
- https://doi.org/10.1094/MPMI.2002.15.4.360