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CRISPR-induced exon skipping is dependent on premature termination codon mutations
- Source :
- Genome Biology, Vol 19, Iss 1, Pp 1-5 (2018)
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Abstract In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing.
- Subjects :
- Exon skipping
PTC
CRISPR/Cas9
Rabbits
Biology (General)
QH301-705.5
Genetics
QH426-470
Subjects
Details
- Language :
- English
- ISSN :
- 1474760X
- Volume :
- 19
- Issue :
- 1
- Database :
- Directory of Open Access Journals
- Journal :
- Genome Biology
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.227e3bdccb8e41f8a1375b16c41e6823
- Document Type :
- article
- Full Text :
- https://doi.org/10.1186/s13059-018-1532-z