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Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin

Authors :
Dušan Sredojević
Vesna Lazić
Andrea Pirković
Jovana Periša
Natalija Murafa
Biljana Spremo-Potparević
Lada Živković
Dijana Topalović
Aleksandra Zarubica
Milica Jovanović Krivokuća
Jovan M. Nedeljković
Source :
Nanomaterials, Vol 12, Iss 18, p 3195 (2022)
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control.

Details

Language :
English
ISSN :
20794991
Volume :
12
Issue :
18
Database :
Directory of Open Access Journals
Journal :
Nanomaterials
Publication Type :
Academic Journal
Accession number :
edsdoj.2213caf201145aea66c76b55658cd30
Document Type :
article
Full Text :
https://doi.org/10.3390/nano12183195