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Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triple-negative Breast Cancer Cells

Authors :
MENG Lingjiao
DING Ping'an
JU Yingchao
LIU Sihua
CHANG Sheng
HAN Ruoqi
PAN Shuo
LIU Fei
GU Li'na
SANG Meixiang
Source :
Zhongliu Fangzhi Yanjiu, Vol 46, Iss 3, Pp 233-238 (2019)
Publication Year :
2019
Publisher :
Magazine House of Cancer Research on Prevention and Treatment, 2019.

Abstract

Objective To investigate the expression of circular RNA ciRS-7 in triple-negative breast cancer (TNBC) and its effect on invasion and migration. Methods The expression levels of ciRS-7 in TNBC tissues and cells were detected by qRT-PCR, and its relationship with clinicopathological parameters of breast cancer patients was further analyzed. Scratch and Transwell assays were used to detect the migration and invasion of MDA-MB-231 and BT-549 cells after silencing ciRS-7. Animal experiment was performed to verify the above findings in vivo. Results The relative expression of ciRS-7 in TNBC tissues was (6.52±0.38), significantly higher than Luminal type (1.56±0.17) (P < 0.001), HER2 overexpressing breast cancer tissues (2.27±0.66) (P < 0.001) and normal adjacent tissues (0.83 ± 0.09) (P < 0.001). Clinical data analysis showed that ciRS-7 expression was significantly associated with molecular typing, tumor invasion and lymph node metastasis (all P < 0.05), not associated with patients' age, tumor diameter or pathological grade (all P > 0.05). After silencing ciRS-7, the migration and invasion of MDA-MB-231 and BT-549 cells were significantly reduced (all P < 0.05). The number of lung metastasis clones was significantly reduced in ciRS-7 knockdown group (P < 0.05). Conclusion ciRS-7 is highly expressed in TNBC and may enhance the invasion and migration of TNBC cells.

Details

Language :
Chinese
ISSN :
10008578
Volume :
46
Issue :
3
Database :
Directory of Open Access Journals
Journal :
Zhongliu Fangzhi Yanjiu
Publication Type :
Academic Journal
Accession number :
edsdoj.20f10e4d908a4fedb5d1f36c0e081acb
Document Type :
article
Full Text :
https://doi.org/10.3971/j.issn.1000-8578.2019.18.0855