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Scalable purification of extracellular vesicles with high yield and purity using multimodal flowthrough chromatography

Authors :
Scott E. Bonner
Simonides I. van deWakker
William Phillips
Eduard Willms
Joost P. G. Sluijter
Andrew F. Hill
Matthew J. A. Wood
Pieter Vader
Source :
Journal of Extracellular Biology, Vol 3, Iss 2, Pp n/a-n/a (2024)
Publication Year :
2024
Publisher :
Wiley, 2024.

Abstract

Abstract Extracellular vesicles (EVs) are cell derived membranous nanoparticles. EVs are important mediators of cell–cell communication via the transfer of bioactive content and as such they are being investigated for disease diagnostics as biomarkers and for potential therapeutic cargo delivery to recipient cells. However, existing methods for isolating EVs from biological samples suffer from challenges related to co‐isolation of unwanted materials such as proteins, nucleic acids, and lipoproteins. In the pursuit of improved EV isolation techniques, we introduce multimodal flowthrough chromatography (MFC) as a scalable alternative to size exclusion chromatography (SEC). The use of MFC offers significant advantages for purifying EVs, resulting in enhanced yields and increased purity with respect to protein and nucleic acid co‐isolates from conditioned 3D cell culture media. Compared to SEC, significantly higher EV yields with similar purity and preserved functionality were also obtained with MFC in 2D cell cultures. Additionally, MFC yielded EVs from serum with comparable purity to SEC and similar apolipoprotein B content. Overall, MFC presents an advancement in EV purification yielding EVs with high recovery, purity, and functionality, and offers an accessible improvement to researchers currently employing SEC.

Details

Language :
English
ISSN :
27682811
Volume :
3
Issue :
2
Database :
Directory of Open Access Journals
Journal :
Journal of Extracellular Biology
Publication Type :
Academic Journal
Accession number :
edsdoj.20762f9074cf6b277f96e8773807b
Document Type :
article
Full Text :
https://doi.org/10.1002/jex2.138