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Uncertainty evaluation for determination of anesthetics residues and their metabolites in aquatic products by high-performance liquid chromatography-tandem mass spectrometry

Authors :
ZHANG Luwen
XIA Sujie
GU Zhengjian
CHEN Yan
PAN Ying
Source :
Shanghai yufang yixue, Vol 34, Iss 6, Pp 533-540 (2022)
Publication Year :
2022
Publisher :
Shanghai Preventive Medicine Association, 2022.

Abstract

ObjectiveThe study aimed to evaluate the measurement uncertainty in the determination of five cacaine anesthetics and their metabolites residues by high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS).MethodsThrough the establishment of mathematical model, the sources of uncertainty were analyzed, and various components were quantified and synthesized to evaluate the influence of the uncertainty components on the measurement results.ResultsThe uncertainties of the experiments were mainly derived from calibration curve fitting, sample pretreatment, recovery rate, standard solution preparation and measurement repeatability. Furthermore, the expanded uncertainty related to the content in shrimp (k=2) was 1.18 μg‧kg-1 at 8.68 μg‧kg-1 for tricaine methanesulfonate (MS-222), 1.28 μg‧kg-1 at 9.11 μg‧kg-1 for benzocaine, 13.5 μg‧kg-1 at 91.4 μg‧kg-1 for 4-aminobenzoic acid, 12.2 μg‧kg-1 at 91.0 μg‧kg-1 for p-acetylamino benzoic acid, and 11.3 μg‧kg-1 at 95.3 μg‧kg-1 for 3-aminobenzoic acid, respectively.ConclusionThis method is suitable for the uncertainty analysis of cacaine anesthetics and their metabolites determination in aquatic products by LC-MS/MS, and can provide scientific and reliable basis for the measurement accuracy.

Details

Language :
Chinese
ISSN :
10049231
Volume :
34
Issue :
6
Database :
Directory of Open Access Journals
Journal :
Shanghai yufang yixue
Publication Type :
Academic Journal
Accession number :
edsdoj.203ec138fba4f7b8c0114535659f592
Document Type :
article
Full Text :
https://doi.org/10.19428/j.cnki.sjpm.2022.22042