Regulation of non-classical immune parameters in immune thrombocytopenic purpura mice by a spleen-invigorating, qi-replenishing and blood-containing formula

Objective: This study investigated the regulatory effect of non-classical immune parameters on immune thrombocytopenic purpura (ITP) mice by a spleen-invigorating, qi-replenishing and blood-containing formula (SQBF). Method: A total of 80 BALB/c mice were randomly divided into four equal groups (20 mice each): control group, model group, prednisone group and spleen-invigorating, qi-replenishing and blood-containing (SQBF) group. Mice in the model group, prednisone group, and SQBF group were administered anti-platelet serum to induce ITP. The dynamic variations of platelet counts in ITP mice were measured with an automatic blood analyzer before modeling and 48 h, and 8, 12 and 15 days following APS injection. Levels of β-endorphin (β-EP), vasoactive intestinal peptide (VIP) and salivary IgA (SIgA) were detected by enzyme-linked immunosorbent assay (ELISA) on 15th day of experiment. Results: SQBF enhanced peripheral blood platelet counts in ITP mice similar to that of prednisone, and both groups showed a statistically significant response compared with the model group (P