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Transcriptional Landscape of Staphylococcus aureus Kayvirus Bacteriophage vB_SauM-515A1

Authors :
Maria Kornienko
Gleb Fisunov
Dmitry Bespiatykh
Nikita Kuptsov
Roman Gorodnichev
Ksenia Klimina
Eugene Kulikov
Elena Ilina
Andrey Letarov
Egor Shitikov
Source :
Viruses, Vol 12, Iss 11, p 1320 (2020)
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

The Twort-like myoviruses (Kayvirus genus) of S. aureus are promising agents for bacteriophage therapy due to a broad host range and high killing activity against clinical isolates. This work improves the current understanding of the phage infection physiology by transcriptome analysis. The expression profiles of a typical member of the Kayvirus genus (vB_SauM-515A1) were obtained at three time-points post-infection using RNA sequencing. A total of 35 transcription units comprising 238 ORFs were established. The sequences for 58 early and 12 late promoters were identified in the phage genome. The early promoters represent the strong sigma-70 promoters consensus sequence and control the host-dependent expression of 26 transcription units (81% of genes). The late promoters exclusively controlled the expression of four transcription units, while the transcription of the other five units was directed by both types of promoters. The characteristic features of late promoters were long -10 box of TGTTATATTA consensus sequence and the absence of -35 boxes. The data obtained are also of general interest, demonstrating a strategy of the phage genome expression with a broad overlap of the early and late transcription phases without any middle transcription, which is unusual for the large phage genomes (>100 kbp).

Details

Language :
English
ISSN :
19994915
Volume :
12
Issue :
11
Database :
Directory of Open Access Journals
Journal :
Viruses
Publication Type :
Academic Journal
Accession number :
edsdoj.1f582c64c61f45479cd3d5052d99988b
Document Type :
article
Full Text :
https://doi.org/10.3390/v12111320