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Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers

Authors :
María Pedrero
F. Javier Manuel de Villena
Cristina Muñoz-San Martín
Susana Campuzano
María Garranzo-Asensio
Rodrigo Barderas
José M. Pingarrón
Source :
Biosensors, Vol 6, Iss 4, p 56 (2016)
Publication Year :
2016
Publisher :
MDPI AG, 2016.

Abstract

An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.

Details

Language :
English
ISSN :
20796374
Volume :
6
Issue :
4
Database :
Directory of Open Access Journals
Journal :
Biosensors
Publication Type :
Academic Journal
Accession number :
edsdoj.1ec8274f6e3b4494bfc90b02881c74df
Document Type :
article
Full Text :
https://doi.org/10.3390/bios6040056