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Analysis of ERK3 intracellular localization: dynamic distribution during mitosis and apoptosis
- Source :
- European Journal of Histochemistry, Vol 59, Iss 4 (2015)
- Publication Year :
- 2015
- Publisher :
- PAGEPress Publications, 2015.
-
Abstract
- Extracellular signal-regulated kinases (ERK) 1, 2 and 3 are involved in cell proliferation and differentiation, and apoptosis; although ERK1/2 have been widely studied, limited knowledge on ERK3 is available. The present work aimed at investigating ERK3 distribution during cell cycle and apoptosis in human tumor HeLa cells. The analysis performed by double immunofluorescence and immunoelectron microscopy experiments revealed that during interphase ERK3 is mainly resident in the nucleoplasm in association with ribonuclear proteins involved in early pre-mRNA splicing, it undergoes cell cycle-dependent redistribution and, during apoptosis, it remains in the nucleus in the form of massive nuclear aggregates, then moves to the cytoplasm and is finally extruded.
Details
- Language :
- English
- ISSN :
- 1121760X and 20388306
- Volume :
- 59
- Issue :
- 4
- Database :
- Directory of Open Access Journals
- Journal :
- European Journal of Histochemistry
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.1d9ea6b7c42042608a2b9b7684b9e4c4
- Document Type :
- article
- Full Text :
- https://doi.org/10.4081/ejh.2015.2571